【摘要】 建立了同时测定柑橘中柠檬苦素和诺米林2种苷元的高效液相色谱方法。色谱柱为Phenomenex C18(250mm×4.6mm,5μm),流动相为体积分数45%乙腈,流速为1.0mL/min,柱温为25℃,检测波长在210nm。经考察该方法可行性强,柠檬苦素和诺米林的平均回收率分别99.91%和98.28%,RSD分别为2.55%和2.30%。因此用该法测定了柑橘中的柠檬苦素和诺米林的含量,并取得了理想的结果。更多还原

【Abstract】 High performance liquid chromatography method for the simultaneous determination of two limonoid aglycosides,limonin and nomilin was established.Limonin and nomilin were separated by Phenomenex C18(250×4.6mm,5μm)column with acetonitrile-water,45∶55(V/V)as mobile phase and flow rate was 1mL/min.The column temperature was controlled at 25℃ and the wavelength of detector was set at 210nm.After exploration,the method was feasible,average recovery ratio of limonin and nomilin was 99.91%和98.28% respectively,RSD was 2.55% and 2.30%respectively.Thus,the content of limonin and nomilin in different citrus were determined,and the satisfied results were obtained.更多还原