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啤酒泡沫活性蛋白质中Z4蛋白质的提取及鉴定
Isolation and Identification of Protein Z4 in Beer Foam-positive Protein
【摘要】 主要研究了泡沫活性蛋白质中最重要的Z4蛋白的分离提纯,并利用蛋白免疫印记法(Western Blot)以及质谱(Mass Spectrometry)鉴定Z4蛋白质。此外,还探讨了Z4蛋白质的含量在酿造过程中的变化。实验结果表明,Z4蛋白质的pI值为5.5~6.0,分子质量为43ku,其抗体鉴定Z4有很高的相关性。在酿造过程中,泡沫活性蛋白质发生了很大的变化。
【Abstract】 In this paper,we mainly studied the method of isolation of protein Z4,a foamposirive protein of critical importance in beer,and the application of immunobloting for detection of protein Z4.Furthermore,the change of content of protein Z4 was also analyzed through the process of brewery production.The results showed that the pI value amounted to 5.5~6.0 and its molecular weight is 43000 in protein Z4,which is highly relevant to Z4 antibody indentification.There is great change in protein Z4 through the process of brewery production.
【Key words】 beer; foam-positive protein; protein Z4; Western Blot; Mass Spectrometry;
- 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2007年02期
- 【分类号】TS262.5
- 【被引频次】11
- 【下载频次】248