【摘要】 对灰绿曲霉(Aspergillus glaucus)突变株EU7-22进行单因子和正交实验,确定菌株利用稻草产纤维素酶的液态发酵优化组合条件为:稻草粉3%、蛋白胨0.5%、温度35℃、起始pH 5。在优化条件下培养4~5 d可达到产酶高峰。利用优化培养后的酶液水解稻草粉产糖,在最适条件下,稻草粉酶解反应24 h后,糖化率可达49%。利用稻草粉做底物,EU7-22和产氢克雷伯氏菌HP1联合发酵,1 g稻草粉可产氢23.7 mL。更多还原

【Abstract】 The optimal conditions for cellulase production of Aspergillus glaucus mutant strain EU7-22 was achieved as follows: rice straw powder quantity 3%,peptone quantity 0.5%,original pH 5,and temperature 35℃,by orthogonal experiments.The strain reached its maximum cellulose activity after 4~5 days fermentation.When the cellulase was applied for saccharification of rice straw,a saccharification rate of 49% was obtained after 24h hydrolysis.The saccharification liquid was subsequently used for hydrogen production.A yield of 23.7 mL H2/g rice powder was acquired when using Klebsiella oxytoca HP1 as the hydrogen producer.更多还原