【摘要】 重组N-乙酰鸟氨酸脱乙酰基酶可在重组菌BL21-pET22b-argE中高效表达,大多以不可溶的包涵体形式存在。文中研究了金属离子浓度及添加时间对该菌生长及可溶表达部分酶活力的影响。结果表明,1.0g/L的Mg2+对生物量和酶活有明显促进作用。Co2+的浓度及添加时间不同,对菌的生长及酶活力的影响结果也不同。培养起始加入Co2+会抑制菌的生长及酶活,而在1.0%乳糖诱导2.5h后加入则可解除生长抑制并促进酶活。Ni2+,Cu2+会抑制菌体的生长及酶活。Mn2+、Mo2+和B3+不影响菌的生长,对酶活有促进作用。添加金属离子后,酶活从16.90U/mL提高到251.91U/mL。更多还原

【Abstract】 Recombinant N-acetylornithine deacetylase could be over expressed by recombinant BL21-pET22b -argE. And most of the enzymes were expressed in the form of inclusion bodies. The effects of different metal ions on the biomass and the enzyme activities were studied. The results showed that 1.0 g/L Mg2+ concentration could improve both biomass and enzyme activity greatly. Different concentrations and feeding time of Co2+ would have different influences on biomass and enzyme activity. Contrast to the inhibition of feeding Co2+ at the beginning of the cultivation, adding Co2+ after inducing 2.5h with 1.0% lactose could overcome inhibition on the growth and increase the enzyme activity. Mn2+, Mo2+ and B3+ had no effects on the growth, but could increase NAO activity. Ni2+ and Cu2+ inhibit both biomass and enzyme activity. With the optimal metal ions in the medium, NAO activity reached 251.91 U/mL from the original 16.90 U/mL.更多还原