【摘要】 以5个萝卜品系为材料探讨了不同培养方式、激素与基因型对花药愈伤组织诱导的影响.结果表明固液双层培养较固体与液体培养效果好,0.2mg/L2,4-D与4.0mg/LKT+0.2mg/LNAA对花药愈伤诱导率较高,4.0mg/LKT+0.2mg/LNAA的激素组合愈伤组织生长快,NAA为2.0mg/L时根分化明显,各种激素处理均未见芽的分化.5个品系中NAU-Yb-03与NAU-Dy-13-03对激素反应较为敏感;NAU-Dq-03在所有的处理中均未见愈伤组织的形成.更多还原

【Abstract】 Five advanced lines were employed in researching the effect of different culture methods,hormones and genotypes on the induction of anther calli in radish.The results showed that liquid/solid bi-layer culture medium was better than liquid medium and solid medium,and when the concentration of 2,4-D was 0.2 mg/L or the concentration of KT and NAA was 4.0 mg/L and 0.2 mg/L,respectively,the higher ratio of anther calli induction was obtained.The calli grew fast on the medium supplemented with 4.0 mg/L KT and 0.2 mg/L NAA.The roots obviously differentiated from it when NAA was 2.0 mg/L,but shoots were not obtained in all treatments.Of the five lines,“NAU-Yb-03”and “NAU-Dy-13-03”were more sensitive to hormones than the others,and anther calli could not been induced from all treatments in “NAU-Dq-03”.更多还原