【摘要】 直接用戊二醛固定水样中的浮游病毒,通过超速离心使已固定的浮游病毒沉淀到覆有Formvar膜和碳支持膜的铜网上,经醋酸双氧铀染色后,利用透射电镜对湖水中的浮游病毒进行观察.结果可观察到球形、杆状和蝌蚪状等形态各异的浮游病毒颗粒及球形病毒的囊膜子粒、杆状病毒的核衣壳、具有不同尾部的蝌蚪状病毒等的精细超微结构.从而建立了一种简便、快捷和高效研究浮游病毒的电镜方法.更多还原

【Abstract】 Water samples which contain virioplankton were collected from a freshwater lake,and fixed immediately with glutaraldehyde solution,and then fixed virioplankton were deposited onto mesh Cu electron microscopy grids supported with carbon-coated Formvar film by ultracentrifugation.Grids were stained with uranyl acetate,and examined under transmission electron microscope(TEM).The virioplankton contain morphologically inhomogeneous virions were investigated,e.g.spherical-shaped virus,rod-shaped virus,tadpole-shaped virus.The fine structure,peplomere of spherical-shaped virus,capsid of rod-shaped virus,different form tails,neck region and contracted sheath of tadpole-shaped virus were observed.The data showed that the method of sample preparation and electron microscopic visualization of viroplankton was a simple,fast,effective and valuable technique.更多还原