【摘要】 目的:研究谷氨酸(L-glutamic acid,GLU)及受体阻断剂MK-801对雄性大鼠纹状体(striatum,STR)神经元自发放电活动的影响。方法:应用微电泳方法记录纹状体神经元自发放电活动。结果:微电泳GLU使84.13%STR神经元自发放电频率加快。在43个STR神经元中直接微电泳MK-801可使62.79%STR神经元自发放电频率降低。在微电泳GLU产生兴奋效应的33个STR神经元中,微电泳GLU期间给予MK-801拮抗GLU的兴奋效应,可使90.91%已对GLU产生兴奋效应的STR神经元放电频率减慢。结论:GLU对STR神经元起兴奋作用,但其兴奋作用可被MK-801所拮抗,当直接微电泳MK-801时,可使STR神经元产生抑制效应。研究提示抗谷氨酸作用的药物有抗帕金森病(Parkinson disease,PD)的作用。更多还原

【Abstract】 Objective: The goal of the experiment is to study how L-glutamic acid(GLU) and MK-801 affect spontaneous discharges of the nerve cells in male rat striatum.Methods: Microelectrodes were used to observe the effects of L-glutamic acid(GLU) and MK-801on STR neurons’ spontaneous firing rates.Results: 84.13% of the tested neurons were excited by GLU,With the increase of electrical currents of Micro-electrophoresis,frequency of discharge of STR nerve cells accelerates.Among the 43 STR nerve cells,direct Micro-electrophoresis MK-801 can lower the frequency of discharge of 62.79% STR nerve cells.Of the 33 STR nerve cells on which Micro-electrophoresis GLU has stimulating effect,in the period of Micro-electrophoresis GLU,MK-801 will counter-affect GLU’ stimulating effect and as the result lower the discharging frequency of 90.91% STR nerve cells which has been affected by GLU.Conclusions: GLU has the excitatory effect on STR nerve cells,which,however,may be antagonized.by MK-801;the direct Micro-electrophoresis MK-801 may result in inhibitory effect on the STR nerve cells.The study hints at the function of anti-glutamate medicine restraining Parkinson disease.更多还原