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大熊猫辅酶Q-细胞色素C氧化还原酶的辅酶Q连接蛋白基因cDNA的克隆及序列比较

cDNA cloning and sequences analysis of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein (QP-C) from giant panda

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【作者】 侯万儒陈瑜彭正松吴夏唐宗祥

【Author】 HOU Wanru1,CHEN Yu1,PENG Zhengsong1,WU Xia1,TANG Zongxiang2(1 College of Life Science,China West Normal University,Nanchong 637002,China)(2 College of Agriculture,Sichuan Agricultural University,Ya’an 625014,China)

【机构】 西华师范大学生命科学学院四川农业大学农学院 南充637002南充637002雅安625014

【Abstract】 In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein(QP-C)gene from the giant panda was analyzed.The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues.The pI of the protein is 10.50 and its mole cular weight is 9.60×103 Da.Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site.Comparing the cDNA sequence with amino acid sequence of giant panda’s QP-C gene and cDNA sequence with the amino acid sequence of other species’ QP-C gene shows high similarity.The similarity is 82.3% to 95.2% and 81.5% to 95.1%.

【基金】 国家自然科学基金资助项目(30570275);四川省应用技术项目(2006J13-057);四川省教育厅重点科研项目(2004A101);四川省重点学科建设项目(SZD0420)
  • 【文献出处】 兽类学报 ,Acta Theriologica Sinica , 编辑部邮箱 ,2007年02期
  • 【分类号】Q78
  • 【被引频次】23
  • 【下载频次】139
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