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黑曲霉高产纤维素酶活突变株ZM-8的筛选
Screening of high-yield cellulase mutants of Aspergillus niger ZM-8
【摘要】 对航空诱变的黑曲霉(Aspergillus niger)进行筛选,得到纤维素酶高产突变株ZM-8。以玉米秸秆粉为主要碳源,经固体发酵培养,测得其滤纸酶(FPA)、纤维二糖水解酶(C1)、葡聚糖内切酶(CMCase)、β-葡萄糖苷酶(β-Glase)的酶活力分别为110.2、389.9、489.3U/g和1208.1U/g,比出发菌株各组分的酶活分别提高2.1、3.5、1.7倍和1.8倍。经过5次继代固体发酵试验,证明该菌株具有较好的产酶稳定性。
【Abstract】 A mutant strain ZM-8 with high activity of cellulase was selected from parent strain of Aspergillus niger by treatment of space conditions.Under some conditions,enzyme activities of FPA,C1,CMCase and β-Glase reached 110.2,389.9,489.3 U/g and 1208.1 U/g,compared with parent strain increased 2.1,3.5,1.7,1.8 times .ZM-8 had high stability of cellulase production after transferring for 5 generations and fermentation in solid medium.
【基金】 天水市科委项目,项目编号:2001-03
- 【文献出处】 中国饲料 ,China Feed , 编辑部邮箱 ,2007年05期
- 【分类号】S816
- 【被引频次】55
- 【下载频次】526