【摘要】 目的探讨胶质生长因子(GGF)、增殖细胞核抗原(PCNA)与人垂体腺瘤侵袭性生长的关系。对垂体瘤生长的生化机制作进一步探讨,并对术后生长作预测。方法免疫组织化学方法分析GGF、PCNA在147例垂体瘤中的表达,分析GGF、PCNA的表达与垂体腺瘤侵袭性关系。用GGF干预垂体瘤细胞,观察细胞生长、细胞周期变化情况。结果GGF、PCNA在多数侵袭性垂体瘤阳性表达,侵袭性垂体腺瘤中GGF表达显著大于非侵袭性腺瘤(P<0.05);复发组GGF、PCNA表达明显高于非复发组(P<0.05)。结论GGF可以明显促进垂体瘤细胞生长,与其侵袭性有重要作用。GGF、PCNA能够部分地反映垂体腺瘤细胞的增殖状态及侵袭潜能,可为临床上评价垂体腺瘤侵袭性、复发、治疗及预后提供参考。更多还原

【Abstract】 Objective To explore the relationship between invasion of pituitary adenoma and glial growth factor (GGF) and proliferating cell nuclear antigen (PCNA) and to further explore the biochemical mechanism of pituitary adenoma and evaluate the growth trend of postoperative pituitary adenoma.Methods GGF and PCNA immunohistochemistry were used to analyze proliferation activity in 147 pituitary adenomas. Cells were cultured with GGF to observe cell growing and vascularization .The relationship between proliferation, growth rate and GGF, PCNA expression were evaluated.Results The expression of GGF and PCNA was significantly higher in invasive pituitary adenomas than that of non-invasive pituitary adenomas (P<0.05); the expression of GGF and PCNA was significantly higher in the recurrent group than that in non-recurrent group of invasive pituitary adenomas (P<0.05).Conclusion GGF may partly result in the invasion of pituitary adenoma. GGF and PCNA can partly evaluate the invasion, recurrence and growth trend of postoperative pituitary adenoma.更多还原