【摘要】 胰岛素抵抗是目前众多老年慢性疾病发病的共同途径,其发生机制的研究对于寻找与制定疾病的防治策略最有重要的意义,但传统的研究方案往往局限于某个器官、一货机种代谢产物,或某一、两种基因参与的条件,得出的结论多为局部描述性,难以进行规律上的探素。基于两种组织(肌肉和肝脏)3种不同胰岛素敏感状态下(限食、对照、肥胖)的12组4 040条基因芯片数据,从基因表达数据的统计分析开始,观察不同胰岛素敏感状态下的大鼠组织对糖刺激反应的基因表达情况,比较、阐述胰岛素抵抗发生的分子机理;并通过SOM聚类分析的方法,发现不同胰岛素敏感状态下的大鼠基因表达变化下所隐藏的表达模式,从而为进一步分析胰岛素抵抗发生的分子机理提供了可能。更多还原

【Abstract】 Insulin resistance is a common pathogenesis of many aged chronic diseases.To understand the pathogenesis is very important.Traditional research on insulin resistance is limited to a special apparatus,a kind of metabolizing outcome,or some kinds of methods which only one or two gene is concerned with,and most of them only achieve some kind of descriptive conclusion.Those methods did not integrate with multi-dimensional metabolic cross talking,considered little of the molecule biology background,and especially lacked of disciplinarian exploring under the huge data space.Based on the 12 groups gene expression data of two tissues of the rats under different insulin sensitivity status(restrict,compare and obesities),a gene expression processing and analysis system is built,the functional of the system includes,the description of the change of the two main rats tissues are observed which make use of dextrose,the expression pattern of gene expression profile is found by the SOM cluster algorithm,and makes it possible to discover the molecular mechanism of insulin resistances.更多还原