【摘要】 目的通过对HPLC条件研究,以建立桂枝汤HPLC的定量分析方法。方法采用高效液相色谱法,考察并优化流动相的组成、配比、酸度与流速以及洗脱方法,分离桂枝汤中3味主要药物桂枝、白芍药、甘草的4个指标成分芍药苷、桂皮酸、桂皮醛与甘草酸。结果在色谱柱为μ-BondapakC183.9mm×300mm,10μm;流动相A相为乙腈,B相为0.05%的磷酸水溶液;流速为1.0ml/min;检测波长是267nm及温度为23～26℃的条件下;经45min梯度洗脱,成功的分离分析了桂枝汤中的4个成分。结论方法先进、可靠、简便易行具有应用价值。更多还原

【Abstract】 Objective To study HPLC conditions to establish quantitative analytical method for the “Guizhi Decoction”. Methods The mobile phase of composition, ratio, acidity and flow rate and the mode of elution were investigated to isolate and analyse the four index components(Paeoniflorin, Cinnanic acid, Cinnamaldehydea and Clycyrrihizic acid) in the “Guizhi Decoction”. Results The optimum condition was μ-Bondapak C18 column with acetonitrile-water-0.05% phosphonic acid gradient elution system, 1.0ml·min-1 flow rate, 267 nm. detection wavelength, and 23～26 ℃ temperature. The analysis ended within 45 min. Conclusion This method is advanced, reliable, simple and practically valuable.更多还原