【摘要】 将编码猪细小病毒主要免疫保护性抗原VP2基因插入干酪乳杆菌细胞表面表达载体pPG中,构建了重组表达载体pPG-VP2,将其电转化干酪乳杆菌Lactobacillus casei 393,获得了表达猪细小病毒VP2蛋白的重组干酪乳杆菌系统,经2%乳糖在MRS培养基中的诱导表达,SDS-PAGE检测表明,有约74kD蛋白得到了表达,表达蛋白的大小与理论值相符。Western-blot结果分析表明,表达的蛋白可被鼠源PPV抗血清所识别,间接免疫荧光实验结果表明,所表达的蛋白能够在干酪乳杆菌菌体表面检测到。更多还原

【Abstract】 Lactobacillus casei 393 was selected as a bacterial carrier for the ex pression of Porcine Parvovirus(PPV)protective antigen VP2 protein.The gen e en coding PPV VP2 protein was cloned into the Lactobacillus casei surface expre ssio n vector pPG,and then the constructed recombinant vector pPG-VP2 was electrotr a nsformed into Lactobacillus casei 393 generating the recombinant system pPG-VP2/L.casei393 expressing PPV VP2 pr otein.The recombinant stra i n was induced by 2% Lactose in MRS and about 74kD protein was detected with SDS-PAGE.The result of Western-blot indicated that the expressed protein possessed the antigenic specificity which could be recognized by mouse anti-PPV serum.Th e indirect immunofluorescent test showed that the expressed protein was secreted on the cell surface Lactobacillus casei.更多还原