【摘要】 目的测试音乐和彩光干预对改善制动小鼠福利的效果,以筛选合适干预手段。方法以反复制动造成小鼠轻度福利损害,分别用进行曲、小夜曲、红色光照和蓝色光照进行同步干预,记录其实验期间的饲料消耗和体增重情况,测定饲料功效比(FER)、生理指标[体重、胸腺指数、脾脏指数、外周血白细胞数(WBC)]和生化指标[血清中促肾上腺皮质激素(ACTH)、皮质酮(CORT)、β-内啡肽(β-EP)、肾上腺素(EPI)、去甲肾上腺素(NE)、白介素-2(IL-2)、总蛋白(TP)、血糖(GLU)和总胆固醇(TCHO)含量]。结果制动使小鼠生长受抑,FER、WBC、IL-2、β-EP(P<0.01)和脾脏指数(P<0.05)降低,ACTH、CORT、NE(P<0.01)和EPI(P<0.05)升高。与模型对照组相比,蓝色光照和小夜曲均能降低制动小鼠的ACTH和NE水平(P<0.01),蓝色光照组小鼠的β-EP水平(P<0.01)、IL-2水平、外周血白细胞数(P<0.01)和胸腺指数(P<0.01)增高(与空白对照组比)。红色光照组小鼠白天相对摄食更多,进行曲干预小鼠皮质酮水平过度下降。结论蓝色光照干预和小夜曲干预均能减轻制动小鼠的神经内分泌反应,蓝色光照还有助于改善制动小鼠的免疫抑制状况,提示两者可以在一定程度上提升小鼠对抗制动的能力,对制动小鼠福利具有较明显的改善作用。更多还原

【Abstract】 Objective To investigate the positive effects of four different forms of environmental enrichment on the welfare of mice subjected to restraint stress with the purpose of screening suitable enrichment. Method Mice undergoing poor welfare due to repeated restraint were kept in different enriched environment (enriched by march, serenade, blue filter or red filter), food consumption and body weight gain were recorded throughout the study. Two weeks later all the animals were assessed for their stress level by measuring physiological parameters (body weight, food efficiency ratio[FER], thymus index, spleen index and total leukocyte in peripheral blood[WBC]) and biochemical parameters (serum levels of ACTH, CORT, β-EP, EPI, NE, IL-2, TP, GLU, TCHO). Result In restraint control group, restraint stress decreased their body weight gain, FER, WBC, IL-2,β -EP(P<0.01) and spleen index (P<0.05), while increased their ACTH,CORT, NE(P<0.01) and EPI(P<0.05). Compared to restraint mice, mice from blue light group and from serenade group exhibit lower levels of ACTH and NE(P<0.01), as well as the restoration level of β -EP(P<0.01) by blue light, indicating elevated neuroendocrine responses to restraint stress. With respect to the higher level of IL-2 (P<0.01), more WBC(P<0.01) and larger thymus index(P<0.01, compared to control group), blue light may contribute to relieve the inhibi- tory status in immune system induced by restraint. When comes to other groups, mice from red light group tended to eat more at daytime than at night compared to mice from restraint control group, and mice from march reduced their serum levels of CORT too much. Conclusion Enrichment with either serenade or blue filter partly enhanced resistance to repeated restraint stress in mice, thus suggest that both forms of enrichment should be beneficial for the welfare of laboratory mice. However, the effects of march and red light are debatable and need more investigation.更多还原