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嗜水气单胞菌AH-1 quorum sensing负调控Ⅲ型分泌系统的表达
Quorum Sensing Negatively Controls Type Ⅲ Secretion Expression in Aeromonas hydrophila AH-1
【摘要】 通过构建嗜水气单胞菌AH-1 Quorum Sensing(QS)2个关键调节基因ahyI,ahyR的突变菌株,来系统分析嗜水气单胞菌AH-1Ⅲ型分泌系统基因,揭示它们由QS系统调控.在ahyI突变菌中,TTSS分泌效应因子(effector)aexT量显著提高.通过构建LacZ-TTSS基因启动子融合表达,进一步表明QS系统负调控编码TTSS组分的基因.
【Abstract】 A systematic analysis of the typeⅢ secretion genes of Aeromonas hydrophila strain AH-1 by constructing ahyR and ahyI mutant revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the TTSS genes in the presence of lacZ-TTSS gene promoter and the corresponding advanced secretion of AexT in ahyI mutant.
【关键词】 嗜水气单胞菌;
quorum sensing;
Ⅲ型分泌系统;
负调控;
【Key words】 Aeromonas hydrophila; Quorum Sensing; typeⅢ secretion system (TTSS); negatively control;
【Key words】 Aeromonas hydrophila; Quorum Sensing; typeⅢ secretion system (TTSS); negatively control;
【基金】 国家自然科学基金资助项目(30370030)~~
- 【文献出处】 生物化学与生物物理进展 ,Progress in Biochemistry and Biophysics , 编辑部邮箱 ,2007年06期
- 【分类号】Q78
- 【被引频次】7
- 【下载频次】251