【摘要】 以大孔吸附树脂提取的大豆皂苷粗提物为研究对象,利用阳离子交换树脂进行纯化。以HPLC分析方法,苷元显色法等多种分析手段,检测树脂纯化的除杂和脱色能力,验证此方法的可行性。通过实验研究,确定了最佳纯化条件:选用50%甲醇水溶液,最佳pH为6.0。本法除杂率达33.01%,脱色率达52.36%。此法不仅有效地去除了样品中的色素等杂质,还可以去除部分大豆异黄酮类物质。更多还原

【Abstract】 A cation exchange resin was applied to purify the crude soyasaponins obtained by solid phase extraction(SPE) using AB-8,a kind of polystyrene matrix macroporous absorbent resins.The purification and decoloration effects were evaluated by means of HPLC and colorimetric determinations.The results revealed that the optimized conditions of the purification procedure as following: 50% methanol aqueous solution with pH=6.0.The results also showed that the method could remove contained impurity substances as high as 33.01% as well as of 52.36% decoloration,the removed impurities are colorants and part of isoflavonoids in dominant.更多还原