【摘要】 选取野桑蚕和5个中系家蚕地理品种为实验材料,采用PCR(polymerase chain reaction)产物直接测序法,获得细胞色素b(cytochrome b,Cyt b)基因5′端591bp的核苷酸序列。对6个核苷酸序列进行变异性分析,并分别利用DNASTAR和PHYLIP软件构建分子进化树。结果表明:所选中系家蚕品种间的序列变异性很低;家蚕品种沿河-1和楚雄是独立起源的;家蚕品种德清-1和延吉的亲缘关系较近。更多还原

【Abstract】 Chinese Bombyx mandarina and 5 chinese B.mori geographical strains were selected as experimental materials.The 5′-end sequences(591bp) of Cyt b gene were gotten by sequencing PCR products directly.The 6 sequences were analyzed and the phylogenic trees were constructed with DNASTAR software and PHYLIP software respectively.The result showed: The sequence divergence of chinese B.mori geographical strains selected was slow;Yanhe-1 and Chuxiong originated independently respectively;B.mori strains Deqing-1 and Yanji were close homogeneous relationship.更多还原