【摘要】 选用健康种猪18头,按1、2、3月龄分为3组,每组6头,无菌采取前腔静脉血10 mL,肝素抗凝,分离外周血单个核细胞(PBMC),计数并稀释成1×10~7个/mL、5×10~6个/mL、2．5×10~6个/mL、1．25×10~6/mL、6．25×10~5个/mL五个浓度梯度;植物血凝素—p(PHA—p)配成100μg/mL、50μg/mL、25μg/mL、12．5μg/mL、6．25μg/mL五个浓度梯度。采用正交试验法在96孔细胞培养板中加入不同浓度的PBMC和PHA—p,用MTT法检测淋转试验结果。三组试验结果均表明PBMC和PHA—p对猪淋巴细胞增殖反应均有显著的影响(0．01＜p＜0．05),其最佳反应条件组合为:PBMC浓度为1．25×10~6个/mL,PHA—p浓度为12．5μg/mL,同时也表明年龄大小不影响猪的淋转试验。更多还原

【Abstract】 The paper choices 18 caput of health boardistinguishedinto 3 groups by different month(one month,two month, three month).Every group includ 6 caput of health boar;asepsis adopting blood of vein 10ml in former celiac,adding anticoagulant,separating peripheral blood monocyte(PBMC),taking count of PBMC and diluting different 5 grads of chroma (1×10~7 individual/ml,5×10~6 individual/ml,2.5×10~6 individual/ml,1.25×10~6 individual/ml,6.25×10~5individual/ml);plant prothrombin-p(PHA-p)was matched different 5 grads of chroma(100μg/ml,50μg/ml,25μg/ml,12.5μg/ml,6.25μg/ml). Adopting orthogonal test PBMC and PHA-p of different chroma entered into 96 apertures cell-cultivated board,detecting result of lymph transform test by means of MTT.3 groups of test result indicate:PBMC and PHA-p significantly influenced increment reactivity of lymphocyte of pig(0.01＜p＜0.05),that optimal condition of reactivity:chroma of PBMC is 1.25(?)~6 individual/ml,chroma of PHA-p is 12.5μg/ml,simultaneity,age magnitude didn’t influence lymph transform test of pig.更多还原