【摘要】 对7株赤潮原甲藻28S rDNA 5′端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.更多还原

【Abstract】 The 28S rDNA partial sequences of several strains of Prorocentrum were cloned and sequenced,and other 14 corresponding sequences of Prorocentrum were obtained from Genbank.Then the phylogenetic trees of Prorocentrum were constructed with the methods of NJ(neighbour-joining) and ME(minimum evolution).The results demonstrated that the sequenced partial 28S rDNA of 7 strains was 950~958 bp.The phylogenetic tree based on the NJ method was similar with that based on the ME method.The sequences of the different strains within the same species are highly conservative;otherwise,the diversity among the sequences from different species was obvious.P.micans separated from the South China Sea was very different from the P.micans strains separated from other area of the world,and the distances between them were even longer than the distances between two species.The sequences from the 28S rDNA would be helpful to design species-specific probes of the phytoplankton.更多还原