【摘要】 目的检测光化学损伤后视网膜色素上皮(RPE)细胞中促红细胞生成素(EPO)及其受体(EPOR)的表达,并探讨其意义。方法成人ARPE-19细胞株传代培养2~5代,建立光损伤模型,分别于光照后6、12和24h终止培养,采用免疫组化SP法和W estern b lot免疫印迹法检测EPO和EPOR在RPE细胞中的表达。结果EPO、EPOR表达均于培养终止后12 h达到高峰,24 h开始下降。结论EPO及其受体EPOR在光损伤RPE细胞中表达升高;这可能是光化学损伤时RPE细胞的自我保护性机制之一。更多还原

【Abstract】 [Objective]To assess the expression and significance of erythropoietin(EPO)and erythropoietin receptor(EPOR) in human retinal pigment epithelial(RPE)cells exposed to light.[Methods]The 2~5 generation cultured human RPE cells were exposed to light of(2000±500) lux energy for 12 h to establlsh light induced injury model,and the culture was finished after 6、12 and 24 h respectively.Immunocytochemical staining and Western blot were used to determine the expression of EPO and EPOR in human RPE cells.[Results]In the 12 h treated group,the expression of EPO and EPOR reached the peak,and began to decrease after 24 h.[Conclusion]Expression of EPO and EPOR in human RPE cells can be promoted by light-induced injury.It may be one of the protective mechanisms of RPE cells in injured by light.更多还原