【摘要】 目的探讨充血性心力衰竭(CHF)患者自然杀伤细胞(NK)活性的变化。方法应用流式细胞仪测定CHF患者全血NK细胞数目,酶联免疫吸附法测定血清、培养上清液中白细胞介素6(IL-6)含量,改良MTT法测定NK细胞杀伤活性。结果CHF患者循环NK细胞数目和NK细胞杀伤活性显著降低,并与心衰程度呈负相关(P均<0.01);CHF患者血清、培养上清液和IL-2刺激后上清液中IL-6显著增加(P均<0.01),并与心衰程度呈正相关(P均<0.01)。结论NK细胞数目和活性下降可能是CHF患者免疫功能异常的机制之一。更多还原

【Abstract】 [Objective] To explore the change and significance of activity of natural killer cells in patients with congestive heart failure(CHF).[Methods] 30 normal controls and 48 patients with CHF were recruited according to NYHA standard.Levels of circulating NK cells were determined by flow cytometry.IL-6 in sera and in supernatant of PBMC cultured in vitro was quantitated by an enzymelinked immunosorbent assay.NK cells killing activity was examined with modified MTT approach.[Results] Compared with normal control group,circulating NK cells and NK cells activity in patients with CHF decreased significantly(P<0.01),and negatively correlated with the degree of heart failure(P<0.01).The levels of IL-6 in sera,in culture supernatant,and in culture supernatant stimulated with IL-2 were higher(P<0.01).There is positive correlation with the degree of heart failure(P<0.01).Cytolytic function of NK cells in patients with CHF increased significantly after being stimulated with IL-2(P<0.01).[Conclusion] The decrease of NK cells may be one of the mechanism of dysfunction of immune system in patients with CHF.更多还原