【摘要】 目的研究ST I571诱导K 562细胞耐药前后基因差异表达情况,探讨其耐药机制。方法用药物浓度逐步递增的方法诱导野生型K 562细胞(K 562-W)对ST I571产生耐药,用台盼蓝染色、M TT法对耐药K 562细胞(K 562-R)进行特性鉴定。应用DNA芯片技术对ST I571耐药前后的K 562细胞的基因差异表达进行检测。结果诱导出K 562-R的耐药浓度为0.5μm ol/L,活细胞比例为98.5%。芯片结果发现662个基因出现差异表达,其中335个基因表达上调,327个基因表达下调。结论利用DNA芯片技术挑选与耐药可能相关的基因,可为ST I571耐药机制的研究提供新的切入点。更多还原

【Abstract】 [Objective] To study the different gene expression profile of K562 cells resistance to STI571 and to investigate the potential mechanism of acquired resistance.[Methods] The wild-type K562 cells were cultured in gradually increased concentrations of STI571.Trypan blue dye and MTT assay were applied to analyses the characteristics of the resistant K562 cells(K562-R).The gene expression difference between K562-R and K562-W was examined with cDNA array.[Results] The survival percentage of K562-R was 98.5% by Trypan blue dye and MTT assay confirmed K562-R can live steadily at 0.5 μmol/L STI571.We detected different expression of 662 genes in which 335 genes were up-regulated and 327 genes were down-regulated.[Conclusion] To identified genes related to STI571 resistance with cDNA array would provide a way on the potential mechanism of STI571 resistance.更多还原