【摘要】 目的:探讨鼻腔吸入r干扰素(IFN-r)对变应性鼻炎(allergic rhinitis,AR)的防治作用。方法:采用卵清蛋白(OVA)、氢氧化铝建立大鼠AR模型。IFN-r鼻腔吸入组(C组)与模型建立第23~30天,每日1次鼻腔吸入IFN-r 12μg,第31天取鼻腔灌洗液测定细胞成分,白介素(IL)-4,IL-5浓度,取血测定血浆IgE水平。设正常对照组(A组),AR模型磷酸盐缓冲液(PBS)处理组(B组)和丙酸倍氯米松吸入组(D组),每组5只大鼠。结果:C组鼻腔灌洗液中的嗜酸性粒细胞(EOS)为(0.005±0.003)×104/ml,明显低于B组的(0.223±0.068)×104/ml(P<0.01);C组鼻腔灌洗液中的IL-4为(7.8±3.2)pg/mL,IL-5为(12.5±4.6)pg/mL,均明显低于B组(P<0.01);C组血浆中总IgE为(38.5±9.6)μg/ml,OVA特异性IgE为(19.6±5.2)U/mL,明显低于B组(P<0.01)。结论:鼻腔吸入IFN-r可以抑制AR鼠IL-4和IL-5的合成,抑制EOS在鼻腔内的炎性浸润及血浆中总IgE和OVA特异性IgE水平,因而,对AR具有一定的防治作用。更多还原

【Abstract】 Objective: To investigate the efficacy and mechanism of nebulized interferon gamma(IFN-r) in the prevention and treatment of allergic rhinitis.Methods: Ovalbumirl(OVA) absorbed to aluminum hydroxide was used to construct the allergic rhinitis model(group C),and nebulized IFN-r 12 ug was inhaled once daily on day 23 to day 30.The nasal lavage fluid was collected on day 31,and the cellular constituents,levels of IL-4,IL-5 and IgE were determined.The normal control group(group A),the allergic rhinitic model group(group B) and beclomethasone dipropionate group(group D) consisted of five mice each.Results: Decrease of eosinophils(0.005±0.003)×104/ml was seen in nasal lavage fluid of group C as compared with group B[(0.223±0.068)×104/ml,(P<0.01)],and the levels of IL-4(7.8±3.2 pg/mL) and IL-5(12.5±4.6pg/mL) decreased significantly in comparison with group B(P<0.01).The serum levels of total IgE(38.5±9.6ug/ml) and ovalbumin-specific IgE(19.6±5.2U/mL)decreased significantly in comparison with those of group B(P<0.01).Conclusion: IFN-r can inhibit the composition of IL-4 and IL-5,and inhibit the airway inflammation with eosinophilic infiltration and the serum levels of total IgE and ovalbumin specific IgE,and so it is helpful in prevention and treatment of allergic rhinitis.更多还原