【摘要】 目的观察黄芪碱化合物HDTIC-1和2对人胚肺二倍体成纤维细胞(2BS)p16、p21表达的影响,以探讨其延缓复制性衰老的分子机制。方法用RT-PCR、Western blot法分析HDTIC化合物培养的2BS细胞及对照细胞p16、p21表达水平。结果1)HDTIC培养的2BS细胞在45代龄未见p16表达,在56代龄p16虽在mRNA水平有弱表达,但在蛋白质水平未见表达,而老龄对照细胞p16表达显著;2)HDTIC培养的2BS细胞在中年代龄p21有弱表达,在老龄细胞p21表达明显,HDTIC培养的2BS细胞与对照细胞p21表达水平差异无统计学意义。结论HDTIC化合物对2BS细胞p21表达无显著影响,但显著抑制p16的基因表达,这可能是HDTIC化合物延缓2BS细胞复制性衰老的主要分子机制之一。更多还原

【Abstract】 Objective To investigate the effects of HDTIC-1 and HDTIC-2 isolated from Astragali Radix on the expression of p16 and p21 of human fetal lung diploid fibroblasts(2BS) so as to explore the mechanism of HDTIC delaying replicative senescence. Methods To analyse the expression levels of p16 and p21 of 2BS cells cultured with DMEM containing HDTIC by RT-PCR and western blot. Results 1)There was no expression of p16 in PD 45 2BS cells incubated with HDTIC.In PD 56 2BS cells cultured with HDTIC, there was a weak mRNA expression of p16, whereas, the protein expression of p16 was not observed.However,an obvious expression of p16 was seen in the control senescent cells. 2)For the cells cultured with HDTIC,there was a weak expression of p21 in the middle age and a strong expression of p21 in the senescent cells.Additionally,no difference of p21 level between the HDTIC-cultured cells and the control cells was seen. Conclusion There was no effect of HDTIC compounds on the expression of p21.But p16 expression of 2BS cells was strongly inhibited by HDTIC,which may contribute to their delaying replicative senescence.更多还原