【摘要】 目的:探讨复方水蛭素抗肿瘤的作用机理。方法:①对W256肿瘤的影响:于大白鼠左前腋下接种W256瘤种细胞液(0.3 ml/只),次日开始用药。阴性对照组给予生理盐水;阳性对照组给予5-FU;中药组分别给予不同剂量的复方水蛭素;②对胃癌靶细胞(NKM)的影响:将药物稀释成1∶10、1∶20、1∶40、1∶80、1∶160、1∶320、1∶640的浓度,接种于多孔培养板内的人胚胎肺细胞(HEL)和肿瘤靶细胞(NKM)上,37℃、CO2培养,观察细胞病变,计算抑瘤指数;③对超氧化物歧化酶(SOD)和脂质过氧化物(MDA)的影响:取小鼠44只,随机分成四组,给药方法同W256肿瘤实验组,连续用药30 d,次日摘取小鼠眼球取血,用微量快速检测法测定SOD和MDA的含量。结果:复方水蛭素对W256肿瘤具有明显的抑制作用(P<0.01),该药浓度在1∶80(最大抑瘤浓度)时抑制肿瘤靶细胞的效应最大,1∶640(最小抑瘤浓度)时,抑制肿瘤靶细胞的效应最小。同时,复方水蛭素还能明显提高小鼠血中SOD的活性和降低MDA代谢产物的含量(P<0.05)。结论:复方水蛭素具有明显的体内外抗肿瘤作用。更多还原

【Abstract】 Objective: To investigate the anti-tumor mechanism of hirudin.Methods: ①W256 tumors were transplanted into the left armpit of rats.Then they were divided into three groups: the negative control group was subjected to saline,the positive control group was subjected to 5-Fu,and the traditional Chinese medicine group was subjected to compound hirudin.② The drug was diluted to a ratio of 1∶10,1∶20,1∶40,1∶80,1∶160,1∶320,and 1∶640 and was implanted into a porous plate for HEL and NKM under a 37 ℃ and CO2 condition.The inhibition effect was determined.③ 40 mice were also divided into groups as the first experiment. 30 days later,the SOD and MDA contents were determined in the eyeball blood by a trace rapid assay.Results: Compound hirudin effectively inhibited the growth of W256 tumors(P<0.01)and was the most significant at a ratio of 1:80 and was the least significant at a ratio of 1:640.It also can significantly increase SOD activity and reduce MDA metabolity(P<0.05).Conclusion:Compound hirudin can significantly affect the W256 tumor in vivo,the NKM in vitro and the blood SOD and MDA.更多还原