【摘要】 目的:应用新型淋巴内皮标记物D2-40分析甲状腺乳头状癌及周围组织微淋巴管密度(MLVD)与淋巴结转移之间的关系。方法:应用免疫组织化学Envison法检测46例甲状腺乳头状癌和20例结节性甲状腺肿中D2-40和CD34的表达,分别计数MLVD和微血管密度(MVD),分析其与甲状腺乳头状癌淋巴结转移之间的关系,同时与病变中MVD行对比观察。结果:91.3%甲状腺乳头状癌乳头轴心中的间质中无淋巴管。微淋巴管主要位于甲状腺乳头状癌周围结缔组织及被膜中。甲状腺乳头状癌周围结缔组织及被膜中的MLVD和MVD与淋巴结转移有密切关系,MLVD和MVD越高淋巴结转移率越高。同时甲状腺乳头淋巴管内的癌栓与淋巴结转移也显著相关(P<0.001)。结论:甲状腺乳头状癌的转移过程与微淋巴管及微血管的形成有密切关系。干预肿瘤转移抑制血管生成的同时也需要抑制淋巴管生成。更多还原

【Abstract】 Objective:Specimens of papillary thyroid carcinoma and nodular goiter have been immunohistochemically studied by using a novel monoclonal antibody D240 which is specific against lymph epithelial cells in the detection of the newly formed micro-lymphatic vessel density(MLVD) in lesions in order to seek clues of papillary thyroid carcinoma and its well known ability to involve regional lymph nodes.Micro-vascular density(MVD)as the future of tumor angiogenesis was immunochemically compared by CD34.Methods: MLVD and MVD were determined by conventional HE staining and Envison immunohistochemistry using D2-40 and CD34 monoclonal antibodies in 46 specimens of papillary thyroid carcinoma and 20 of nodular goiter.Results: The micro-lymphatic vessel of papillary thyroid carcinoma in the peripheral zone of stroma appeared to be associated with the spread of tumors to regional lymph nodes.Conclusion: The metastasis process of thyroid papillary carcinoma is associated with the formation of the micro-lymphatic vessel and micrangium.The antimetastatic potential for targeting these lymphatics may be of potential therapeutic benefit in the future.更多还原