【摘要】 目的:观察HIF-1α和P-gp在原发性肝癌(HCC)中的表达,探讨两者相互作用的关系。方法:采用免疫组织化学法检测36例HCC及20例非肿瘤肝组织中P-gp和HIF-1α的表达,对两者均表达阳性的病例,进行免疫组织化学双标记染色。结果:36例HCC中HIF-1α和P-gp高表达,阳性率分别为58.33%和77.78%,明显高于非肿瘤肝组织(P<0.001)。两者表达呈正相关(r=0.388,P<0.05)。18例两者均表达阳性的病例中,免疫双标共表达率为61.1%。结论:HCC中HIF-1α和P-gp的表达密切相关,HIF-1α可通过上调P-gp的表达实现对化疗药物的耐受,HIF-1α有望成为评价及逆转肿瘤多药耐药的新指标。更多还原

【Abstract】 Objective:To study the expression of hypoxia induced factor-1α(HIF-1α) and P-gp and their correlation in patients with hepatocellular carcinoma(HCC).Methods: Immunohistochemistry was used to detect the expression of HIF-1α and P-gp in thirty-two HCC samples and twenty noncancerous liver samples.If the expressions of both HIF-1α and P-gp were positive in the same case,another slide was used to detect the co-expression of the two.Results: The positive expressions of HIF-1α(58.33%) and P-gp(77.78%) in HCC were significantly higher than those in the non-cancerous group(P<0.001).The expression of HIF-1α was positively correlated with the expression of P-gp(r=0.388,P<0.05).The co-expression rate was 61.1%.Conclusion: The expression of HIF-1α and P-gp has a close correlation in HCC,and HIF-1α may induce multi-drug resistance by up-regulating the expression of P-gp,and it may become a novel marker to evaluate and reverse multi-drug resistance.更多还原