【摘要】 观察低分子肝素治疗大鼠创伤性深静脉血栓形成的效果,从基因水平探讨低分子肝素治疗创伤性深静脉血栓形成的作用机制。将150只SD大鼠采用定量击打双侧大腿+双后肢石膏固定的方式造模,再将造模后5天有血栓形成的大鼠,随机分为药物治疗组和对照组,分别用低分子肝素和生理盐水进行干预,第一次干预后3h,各组随机取10只大鼠股静脉血管及其主要属支,采用Trizol一步法提取总RNA,运用Gene-chip Rat Genome 4302.0芯片测定股静脉RNA表达。倍数变化分析筛查出差异性表达基因,进一步行path-way分析。结果表明:对照组比较,药物组血栓消退率较高(x2=4.698,P<0.05);有1229个基因呈现差异性表达,该基因主要参与了MAKP、Ca2+、细胞因子及受体信号传导通路,参与MAPK通路的始动基因如IL1、TGF、FGF及其受体,末端效应基因如c-Junn、ur77、p53等均呈现上调。低分子肝素可调控促细胞分裂原活化蛋白激酶(MAPK)信号通路并影响血栓的预后。更多还原

【Abstract】 To observe the curative effects of low molecular weight heparin(LMWH) and to explore the mechanism of lmwh at genic lever in the treatment of rat traumatic deep vein thrombosis(TDVT).150 SD rats were used to establish TDVT model through quantitative beating on the bilateral posterior limbs combined with hip spica cast fixation.Then the rats with TDVT at the 5th day after model establishment were randomly divided into the drug therapy and control groups and treated respectively with LMWH and sodium chloride.At 3h after the primary intervention,femoral vein with its main branches were incised from random 10 rats of all.To adopt to Trizol one-step method for the total RNA extraction of femoral veins.Genechip Rat Genome 430 2.0 genechips were applied for RNA expression detection of femoral vein.Through the fold change analysis,the differential expression genes were selected for pathway analysis.Results showed that compared with the control group,the rate of thrombi solution in therapy group were much higher(x2=4.698,P<0.05).1229 genes presented differential expressions which were involved mainly in MAPK,calcium and cytokine-cytokine receptor interaction pathways.The expressions of priming genes in MAPK pathway,such as IL1,TGF,FGF and their receptors and the expression of end effector genes,such as c-Jun,nur77,p53,etc,were up-regulated.It proves that LMWH can participate in the MAPK pathway regulation and affect the prognosis of TDVT.更多还原