【摘要】 采用沙培的方法,研究了低磷对来自细胞工程的耐低磷玉米自交系SD-C、SD-D和齐319叶片光合作用特征的影响.结果表明:低磷胁迫下,玉米幼苗叶片的无机磷含量、光合速率下降.耐低磷自交系SD-C、SD-D的无机磷含量、净光合速率下降幅度小于齐319.SD-C和齐319叶片光合速率的降低是受非气孔因素的限制,而SD-D则是受气孔因素的限制.低磷处理使玉米叶片PSⅡ的光能转换和利用效率降低,耐低磷玉米自交系SD-C、SD-D的光能转换效率和利用效率高于齐319,受低磷胁迫的影响小于齐319.玉米幼苗叶片内无机磷含量的差异可能是自交系SD-C、SD-D和齐319之间光合作用和叶绿素荧光产生差异的原因.更多还原

【Abstract】 The maize inbred lines SD-C and SD-D regenerated by cell engineering with low level phosphorus tolerance,and inbred line Qi-319(as control) were used to study leaf photosynthetic characteristic under low level phosphorus conditions.The results show that under low phosphorus stress,phosphate content and photosynthetic rate of maize leaves were decreased,reductions of which were less in SD-C and SD-D than in Qi-319.The reduction of the photosynthetic rate might be controlled by non-stomatal limitation in SD-C and SD-D,while by stomatal limitation in 319.Efficiency of light energy conversion and utilization by maize leaves PSⅡ were decreased by low phosphorus stress,which were higher in SD-C and SD-D than in Qi-319.The effect of low phosphorus stress was less in SD-C and SD-D than in Qi-319.The different inorganic phosphorus content in leaves contributed to the variation of photosynthesis and chlorophyll fluorescence in the tolerant maize lines and the control line.更多还原