【摘要】 克隆并测定了四川黑熊(Ursus thibetanus mupinensis)线粒体基因组764 bp的片段,根据序列同源性比较,该DNA片段包括2个蛋白质编码基因:ND3和ND4L基因,以及1个tRNA-Arg基因.四川黑熊的ND3基因和ND4L基因的DNA序列和所编码的氨基酸序列与马来熊、棕熊、美洲黑熊和北极熊的同源性分别为:90%和95%、93%和97%、91%和96%、93%和97%;89%和98.98%、88%和98.98%、89%和100%、89%和98.98%.与棕熊、美洲黑熊和北极熊tRNA-Arg(CGA)的同源性分别为95.65%、94.20%和94.20%.拓扑结构比较显示,四川黑熊的ND3基因所编码的氨基酸序列比美洲黑熊的少了1个蛋白激酶C磷酸化位点.更多还原

【Abstract】 The fragment of mitochondrial genome 764 bp of Sichuan black bear has been cloned and determined.According to homologous comparision,the DNA fragment contains two protein-coding genes(ND3 and ND4L) and one tRNA-Arg gene.The DNA sequence with amino acid sequence of black bear’s ND3 and ND4L gene and the DNA sequence with amino acid sequence of Malay bear’,brown bear’,American black bear’ and polar bear’ ND3 and ND4L gene have the high homology,the homology is 90% and 95%,93% and 97%,91% and 96%,93% and 97%,respeclively;89% and 98.98%,88% and 98.98%,89% and 100%,89% and 98.98%,respeclively.The homology of tRNA-Arg gene with brown bear、American black bear and polar bear’ is 95.65%、94.20% and 94.20%,respeclively.The analysis of topology indicates that ND3 gene of Asian black bear has a less Protein kinase C phosphorylation site than American black bear’s.更多还原