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氧化亚铁硫杆菌突变株对Fe2+及SO32-的氧化研究
Study on the Oxidation of Ferrous Iron and Sulfite by Acidithiobacillus Ferrooxidans Mutant Strain
【摘要】 利用紫外线对氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)进行诱变处理,获得了对SO32-和Fe2+具有较高转化效率的突变菌株。结果表明,突变菌株将Fe2+完全氧化所需时间从诱变前的48 h缩短为24 h,并且具有较宽酸碱度适应范围。在突变菌株与Fe2+共存时,SO32-的氧化速率明显高于化学氧化,且以间接氧化为主,细菌对Fe2+的氧化是反应过程的限制步骤。当SO32-的初始浓度为2.7 g.L-1、Fe2+的浓度为0.43 g.L-1时,SO32-的转化速率为0.0153 g.L-1.min-1。
【Abstract】 Acidithiobacillus ferrooxidans was treated by UV to obtain a mutant strain which has a high conversion efficiency of SO32-and Fe2+.The results showed that the mutant strain reduces the time for the perfect conversion of Fe2+ from 48 hours to 24 hours.The biological oxidation rate of SO32-was obviously higher than the chemical oxidation rate when Fe2+coexisted with the mutant strain.The bacteria did not directly oxidate SO32-,and the oxidization of Fe2+ by the bacteria seemed to be the key step of oxidation of SO32-.The conversion rate of SO32-was0.0153 g·L-1·min-1when the initial concentration of SO32and Fe2+ was 2.7 g·L-1 and 0.43 g·L-1,respectively.
【Key words】 Acidithiobacillus ferrooxidan; mutant strain; ferrous; iron sulfite;
- 【文献出处】 四川大学学报(工程科学版) ,Journal of Sichuan University(Engineering Science Edition) , 编辑部邮箱 ,2007年02期
- 【分类号】X172
- 【被引频次】4
- 【下载频次】184