【摘要】 用0.01、0.10、1.00 mg.L-1久效磷暴露金鱼21 d,测定了精巢乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、碱性磷酸酶(ALP)的活性,并观察了精巢显微和超微结构的变化。结果表明:久效磷暴露21 d,精巢小叶基膜断裂,Leydig氏细胞水肿,小叶间质扩大,支持细胞核膜溶解,胞质内脂滴和髓磷脂象数量增多。1.00 mg.L-1久效磷造成了生殖腺指数的显著下降,对精巢发育有一定程度的延迟作用。随着久效磷暴露浓度的升高,LDH,ACP,ALP的活性逐渐下降,0.10、1.00 mg.L-1久效磷暴露组精巢LDH,ACP的活性下降显著,而各暴露组的精巢ALP活性呈现不显著下降趋势。推测久效磷可能通过损伤精巢超显微结构和降低LDH,ACP和ALP活性,干扰精巢能量代谢,造成对雄性金鱼的生殖毒性。更多还原

【Abstract】 After 21 days’ exposure of adult male goldfish(Carassius auratus) to monocrotophos(0.01,0.10,1.00 mg·L-1),we measured activities of lactate dehydrogenase(LDH),acid phosphatase(ACP),alkaline phosphatase(ALP) in testis and observed micro-ultrastructure of testis by light and electron microscopy separately.It was found that the gonado-somatic(GSI) decreased significantly,ground membrane dissolved,Leydig’s cell swelled and interstitial tissue enlarged,as a result of exposure to monocrotophos at the concentration of 1.00 mg·L-1.Monocrotophos induced dissolution of nuclear membrane of the Sertoli’s cell,and the increase of lipid droplet and myelin-like figure number in Sertoli’s cell.The activities of LDH,ACP and ALP in testis decreased gradually,as the exposure concentration of monocrotophos increased.Exposure to monocrotophos at concentrations of 0.10 and 1.00 mg·L-1 resulted in the decrease of LDH and ACP activities in testis.However,the ALP activity did not decrease significantly at various exposure concentrations.It is supposed that the energy metabolism was interrupted as a result of the changes of micro-ultrastructure and the decrease of LDH,ACP,ALP activities in goldfish testis,which caused the reproductive toxicity effect.更多还原