【摘要】 以贻贝(Mytilus coruscus)为原料制取降血压肽,采用均匀设计试验探讨了不同酶解条件对其ACE(angiotensin-converting enzyme)抑制活性的影响,利用高效液相色谱分析了其相对分子质量分布,以氨基酸自动分析仪分析其氨基酸和游离氨基酸组成;并利用自发性高血压大鼠(SHR)饲喂试验检测其降血压活性。结果表明,对于P1酶来说,酶量从25-175IU.mL-1,酶解时间从2-7h,随着酶解程度的提高,其ACE抑制率下降;其最高的ACE抑制率为88.08%,对应的最佳酶解条件为:酶量25IU.mL-1,酶解时间2h,pH7.0,酶解温度60℃。此外发现降血压肽的相对分子质量在1.6ku以下,其中10肽以下的短肽约占50.4%,证实了降血压肽主要以分子量较小的短肽组成。降血压肽游离氨基含量为8.8g.(100g)-1,水解氨基酸总量为59.6g.(100g)-1,疏水性氨基酸含量为24.86g.(100g)-1,氨基酸组成比例平衡,必需氨基酸含量丰富,其中含量最高的氨基酸是谷氨酸,占8.57%。此外以3.0g.kg-1体重的降血压肽样品剂量饲喂SHR,在饲喂后2-6h降压效果显著,平均降低幅度为18-28mmHg(P=0.05)。研究表明以贻贝等水产蛋白为原料可开发具有高ACE抑制活性和显著降血压作用的降血压肽。更多还原

【Abstract】 The antihypertensive peptides were prepared from Mytilus coruscus,and the effects of different enzymolysis conditions on the ACE inhibitory activities were studied by uniform design experiments.Then the relative molecular weight and amino acids composition were identified by HPLC and amino acid analyzer respectively,and the antihypertensive effects were tested by SHR feeding experiment.The results are as follows: for the enzyme P1,when the enzyme concentration is from 25 to 175 IU·mL-1,and the enzymolysis duration is from 2 to 7 h,the enzymolysis degree is improved,but the ACE inhibitory ratio is reduced.The highest ACE inhibitory ratio is 88.08%,the corresponding optimum enzymolysis condition is: enzyme concentration 25 IU·mL-1,enzymolysis duration 2 h,pH 7.0,enzymolysis temprature 60 ℃.The molecular weight of antihypertensive peptide is below 1.6 ku,the short peptides below decapeptide take 50.4 percent.It is proved that antihypertensive peptide is made up of short peptide with low molecular weight.The free amino acids are 8.8 g·(100g)-1,and the total amino acids are 59.6 g·(100g)-1,the hydrophobic amino acids are 24.86 g·(100g)-1;the composition proportion of amino acids is in equilibrium and the essential amino acids are abundant.The largest content amino acid is glutamic acid,which accounts for 8.57 percent.At last,SHR fed with the antihypertensive peptide with the dose of 3.0 g·kg-1 weight can be found obvious antihypertensive function within 2 to 6 hours,the average reduced breadth is 18-28 mmHg(P=0.05).Results from this study indicated that the antihypertensive peptide with strong ACE inhibitory activities and obvious antihypertensive function can be prepared from Mytilus coruscus,and this study explores a new way of advanced exploitation of marine life resources.更多还原