【摘要】 目的观察酒精诱导大鼠脂肪肝组织中肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)水平的变化。方法以56%酒精灌胃,造成大鼠酒精性脂肪肝模型,取血清测丙氨酸转氨酶(alanine aminotransferase,ALT)、门冬氨酸氨基转氨酶(aspartate aminotransferase,AST)活性,取肝组织做免疫组织化学检测TNF-αI、L-6表达。结果模型组大鼠毛发光泽差,食欲差,大便溏泻,体重增长缓慢(P<0.01),模型组大鼠肝组织出现不同程度的肝脂肪变性,血清中ALT、AST活性较正常组明显升高,差异有统计学意义(P<0.01),模型组肝组织中TNF-αI、L-6蛋白阳性产物呈棕褐色细颗粒状。结论肝细胞中TNF-aI、L-6水平的升高可能是酒精性脂肪肝发生的重要机制之一。更多还原

【Abstract】 Objective To abserve the change of TNF-α and IL-6 in the hepatic tissue of alcoholic fatty liver rats.Methods The rats with alcoholic fatty liver were given 56% alcohol by daily gavage,after 12 weeks,to observer the change of serum activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and the expression tumor necrosis factor(TNF)-α and interleukin(IL)-6 of hepatic tissue were observed.Results The model group rats’livers became more fatty and activities ascending of ALT and AST than normal and there was significant difference(P<0.05).the contents of TNF-α and IL-6 in liver were higher than the normal group rats.Conclusion The increasing of TNF-α and IL-6 in liver tissue is possibly the one of the important mechanism for alcoholic fatty liver.更多还原