【摘要】 为提高几丁质酶产生菌C4的产酶效率,加强其对Bt(Bacillus thuringiensis)的增效作用,本研究通过培养基优化,筛选出了该菌发酵优化培养基,配方为:蛋白胨0.8%,酵母膏0.8%KNO30.8%,MgSO40.05%,KH2PO40.03%,pH 7.5.28℃,180 r/min,培养48 h至对数期,加入1.0%的胶体几丁质,诱导C4菌产生几丁质酶.在此条件下,C4菌产酶周期由5 d缩短为4 d;几丁质酶活力由2.68 U/mL提高到5.95 U/mL.用此发酵菌液与Bt菌液等量混合使用,Bt的杀虫效果提高了47.8%.更多还原

【Abstract】 To improve the chitinase activity of chitinase-producing bcterium C4,a optimal medium was acquired after medium optimization.The optimal conditions were as follows: peptone 0.8%,yeast extract 0.8%,KNO3 0.8%,MgSO4 0.05%,KH2PO4 0.03%,pH 7.5.Adding 1% colloid chitin to the medium to induce the bacterium C4 to produce chitinase.Results showed that: the fermentation period of C4 shortened to 4d and the chitinase activity boost to 5.95 U/mL.Using the mixture of Bt and C4 fermentation broth at the ratio of 1 to 1 to feed silkworm,the mortality rate of Bt bove 47.8% that of using Bt alone.更多还原