【摘要】 以不同Cd2+浓度对土培小麦进行处理,分别在拔节、抽穗、灌浆初期以及灌浆中期,分析不同浓度Cd2+胁迫下活性氧及酶促和非酶促抗氧化系统的变化.结果表明,随着小麦生育进程的变化,Cd2+胁迫使O2.-、H2O2和MDA含量增加,且生育前期的增长量明显大于后期;各生育时期的抗氧化酶和抗氧化物也出现不均衡变化:抽穗—拔节期间,SOD活性升高,CAT保持稳定,酶促抗氧化系统对活性氧的清除作用显著;灌浆期后,SOD、CAT活性均比对照有所下降,而抗坏血酸-谷胱甘肽氧化还原途径中的APX、GR活性以及AsA、GSH含量经前期速降后,逐渐稳定.更多还原

【Abstract】 When wheat was treated with Cd2+ of different concentrations,the changes in enzymatic and non-enzymatic antioxidative system at jointing,heading,early filling and middle filling stage was studied.The result showed that the level of O2·-、H2O2、MDA progressively increased and the increase rate at early developemental stages were significantly higher.The change in actioxidative enzymes and antioxidant were also unbalanced:from jointing stage to heading stage,Enzymatic antioxidative system played an important role on ROS scavenging because of the increase in SOD activity and stability in CAT activity;at filling stage,the activity of SOD and CAT evidently decreased respect to the controls,but the activity of APX and GR,content of AsA and GSH were stabe after significant decrease.更多还原