【摘要】 目的探讨0.4mT工频磁场对中国仓鼠肺成纤维细胞(CHL)微丝(F-actin)装配的影响及作用机制。方法用免疫荧光染色法标记F-actin,激光共聚焦荧光显微镜观察F-actin形态并拍照,用W estern-b lotting方法检测了与去垢剂不可溶的细胞骨架相连的表皮生长因子受体(EGFR)蛋白的含量。结果0.4 mT工频磁场辐照CHL细胞30m in导致细胞F-actin应力纤维减少,在细胞周边出现丝状伪足,磁场对CHL细胞F-actin形态的影响与细胞经50nmol/L的表皮生长因子(EGF)作用30m in类似,且磁场和EGF都使与去垢剂不可溶的细胞骨架相连的EGFR增多。结论0.4mT工频磁场辐照导致CHL细胞F-actin装配发生变化,磁场对F-actin的影响可能与磁场诱导表皮生长因子受体聚集,并引起信号向下传递有关。更多还原

【Abstract】 Objective To investigate the effect of 0.4mT power frequency magnetic field on the microfilament(F-actin) assembly of Chinese hamster lung(CHL) cells.Methods F-actin were marked with immunohistochemical method,then observed under a confocal microscope.The content of EGFRs in the preparation of the detergent-insoluble cytoskeleton was measured with Western-blotting.Results The stress fibers of CHL cells decreased after exposure to 0.4mT power frequency magnetic field for 30min,as well as after treatment with epidermal growth factor(EGF) of 50nM.Filopodias appeared at the periphery after exposure to magnetic field as well as treatment with EGF.The EGF receptor mass associated with the detergent-insoluble cytoskeleton increased after exposure to magnetic field as well as treatment with EGF.Conclusion 0.4mT power frequency magnetic field induced assembly of F-actin in CHL cells.The change induced by magnetic field would be related to clustering of EGFR induced by magnetic field and passing the signal down.更多还原