【摘要】 目的为昆明-无毛鼠(KM-HL)作为白细胞减少症模型实验用鼠提供理论依据。方法分别对KM-HL鼠①用环磷酰胺(Cy);②用137Cs源-γ射线全身一次性照射7.5Gy,剂量率为:0.80645Cy/min致小鼠白细胞减少症,取样对其血液、造血系统、免疫及心脏、肝脏等生理指标的变化情况进行测试研究。结果研究表明正常KM-HL在白细胞减少症的实验中,WBC、DNA、脾脏、胸腺、心脏等一些生理指标与对照组比较都受损较重(P<0.001),但Cy组的肝脏与对照比受损较轻,无统计学意义。结论KM-HL鼠无论用Cy还是用137Cs源-γ射线全身一次性照射7.5Gy,其血液、造血系统、免疫及心脏、肝脏等生理指标与对照组比都有所下降(P<0.001),可做为白细胞减少症的实验用鼠。更多还原

【Abstract】 Objective To provide theoretical foundations for KM-HL mice used as experimental mice on leucopenia model.Methods Cyclophosphamide and 137Cs source γ rays irradiated at 7.5Gy once the whole body(dose rate:0.80645Gy/min)were given on KM-HL mice to induce mice leucopenia model,samples were obtained to examine the variation of physiological indexes such as blood,hematopoietic system,immunity,heart and liver etc.Results The research shows,in normal KM-HL mice in the leucopenia experiment,some of the physiological indexes of WBC,DNA,spleen,thymus,heart shows that they are deeply injured(P<0.001),only the Cyclophospamide group’s liver is lightly injured,without any statistic difference.Conclusion Either giving cyclophosphamide or 137Cs source γ rays irradiating once all over the body,the physiological indexes such as blood,hematopoietic system,immunity,heart and liver etc of KM-HL mice are descended,compared with the control group(P<0.001),therefore it can be used as experimental mice on leucopenia.更多还原