【摘要】 目的探讨脂蛋白脂酶C1573T单核苷酸多态性与有氧耐力训练对心脏结构和功能的影响。方法选择102名从未参加专业训练来自中国北方地区男性士兵作为受试者。对所有受试者运用PCR-RFLP法测定LPL基因内含子6C1573T基因多态性。并对所有受试者进行为期18周的耐力训练,采取递增负荷运动实验测定受试对象训练前后左心室结构和功能。结果3种基因型的分布频率分别为CC:40(0.39),CT:43(0.42),TT:19(0.19)。以单因素方差分析对比不同基因型群心脏结构和功能。训练前3个基因型群间心脏结构和功能的多个指标差异存在显著性(P<0.05)。CT基因型群心脏结构和功能训练敏感性明显大于其他两个基因型群(P<0.05)。结论3个基因型群间心脏结构和功能存在初始遗传水平的差异并显示CT基因型群心脏的结构和功能对运动训练更敏感。更多还原

【Abstract】 Objective To determine the genetic polymorphism of lipoprotein lipase and impact of the results of endurance trains on cardiac structure and function before and after endurance exercise training. Methods The 120 male Han Nationality soldiers from the north of China never participated in professional train were included in the study.The genotypes of intron 6 C1573T in LPL gene of all subjects were determined by PCR-restriction fragment length polymorphism.Leukocyte DNA was extracted by using kit.Amplified DNA was digested with restriction enzymes PvuII.Then three genotypes(CC;CT;TT) were separated based on the restriction site.All subjects were under the training of running 5000 m/times and three times a week with an intensity of 95-105% ventilatory threshold(VT) for a duration of 18 weeks.Left cardiac structure and function were tested under echocardiographic examination and VT were measured on a treadmill before and after the running program. Results The distribution of three genotype frequencies were as follows: CC=0.39,CT=0.42,TT=0.19 and they were coincident with Hardy-Weinberg equilibrium.Cardiac structure and function was significantly different (P<0.05) among various genotype groups in pre-training.The exercise sensitivity of cardiac structure and function in the CT group was significantly higher than the other two groups(P<0.05). Conclusion The original level of heredity of cardiac structure and function was significantly different among various genotype groups and C1573T site of intron 6 polymorphism of the LPL gene,especially CT group,may be a novel genetic marker for cardiac structure and function response to endurance training.更多还原