【摘要】 目的:探讨放射性粒子125I组织间植入诱导H22肝癌细胞凋亡的可能机制及影响。方法:分别把125I、DDP和两者联合植入荷H22肝癌小鼠瘤体内28d,观察肿瘤生长,应用流式细胞术检测离体的H22肝癌细胞凋亡情况。结果:125I、DDP植入肿瘤组织后,瘤体生长受到一定的影响;两者合用后致肿瘤生长变慢且出现变性坏死。细胞凋亡率:125I组为(16.12±1.46)%;DDP组为(10.30±1.96)%;联合组为(6.61±0.56)%,且有较多的死亡细胞。结论:放射性粒子125I组织间植入可诱导肿瘤细胞凋亡,与化疗药合用除诱导肿瘤细胞凋亡,还可导致肿瘤变性、死亡,是一种简便易行,安全有效的新治疗方法。更多还原

【Abstract】 OBJECTIVE:To investigate the effects of H22 hepatoma cells apoptosis induced by radioactive seed 125I. METHODS: 125I, cis-platinum (DDP) and 125I combined with DDP were implanted into the mice H22 hepatoma, individually. After 28 days, the tumor growth was observed, and the apoptosis of H22 hepatoma cells detected in vitro. RESULTS: After 125I, DDP were implanted into the mice H22 hepatoma, the tumor growth showed fast in the first phase and then became slow; 125I combined with DDP made the tumor growth slower, cell degeneration and necrosis. The apoptosis rates of 125I and DDP groups were (16.12±1.46)% and (10.30±1.96)%, individually, and the rate of 125I combined with DDP group was (6.61±0.56)%, and there were more death cells. CONCLUSIONS: The interstitial implant of radioactive seed 125I induces hepatoma cells apoptosis.125I combined with DDP induces apoptosis, cell degeneration and necrosis. The interstitial implant is a new convenient, safe and effective method.更多还原