【摘要】 目的研究乙醇脱氢酶2(ADH2)和乙醛脱氢酶2(ALDH2)基因多态及饮酒习惯与肝癌的易感性。方法对208例原发性肝癌和208例对照调查饮酒习惯,采用RCT-RFLR方法检测ADH2和ALDH2基因型。结果1)病例与对照ADH2和ALDH2基因型分布频率差异均无统计学意义。2)携带AL-DH212或ALDH222基因型且饮酒总量>3kg年者,发生肝癌危险性是携带AL-DH211基因型不饮酒者的3.30倍(95%CI=1.24~8.83);而携带ADH212或ADH222基因型者且饮酒总量>3kg年与携带ADH211基因型不饮酒者相比,患肝癌危险性无显著增加。3)携带AL-DH212或ALDH222同时携带ADH212或ADH222基因型且饮酒总量>3kg年者,与携带ALDH211同时携带ADH211基因型且饮酒总量≤3kg年者相比,患肝癌OR值虽有增加但未达显著性(OR=4.26,95%CI=0.63~29.08)。4)HBsAg阳性并携带ALDH212或ALDH222基因型且饮酒>3kg年者,与HBsAg阴性并携带ALDH211基因型且饮酒≤3kg年者相比,患肝癌危险升高49.71倍(95%CI=5.51~448.96)。结论大量饮酒和肝癌的关联与ALDH2基因有关,而与ADH2基因无关。更多还原

【Abstract】 OBJECTIVE:To investigate the relationship among aldehyde dehydrogenase-2 (ALDH2), alcohol dehydrogenase-2 (ADH2) genotypes and alcohol drinking for the susceptibility of primary hepatocellular carcinoma (PHC). METHODS: A case-control study including 208 cases of PHC and 208 controls was carried out in Taixing Blood samples were collected and tested for ALDH2 and ADH2 genotypes by PCR-RFLP method. RESULTS: 1)The frequencies of ALDH2 and ADH2 genotypes were no significantly different between the PHC cases and controls. 2) Compared with those no-drinkers with ALDH21*1 genotypes, drinkers with ALDH21*2 or ALDH22*2 genotypes and cumulative amount of alcohol consumption >3 (kg*years) were at a significantly higher risk of developing PHC (OR=3.30, 95%CI=1.24-8.83). When compared with those with different ADH2 genotypes, no significant difference was found (OR=1.46,95%CI=0.39-5.47). 3)Drinkers with ALDH21*2 or ALDH22*2 genotypes and ADH21*2 or ADH22*2 genotypes and cumulative amount of alcohol consumption >3 (kg*years) were not at a significantly risk of developing PHC (OR=4.26, 95%CI=0.63-29.08) compared with those no-drinkers with ADH21*1 and ALDH21*1 genotypes. 4)Compared with individuals with ALDH21*1, negative of HBsAg and cumulative amount of alcohol consumption ≤3 (kg*years), those with ALDH21*2 or ALDH22*2, positive of HBsAg and cumulative amount of alcohol consumption >3 (kg*years) were at a significantly higher risk of PHC (OR=49.71, 95%CI=5.51-448.96). CONCLUSIONS: These results reveal that not ADH2 but ALDH2 polymorphisms have a significantly interaction with heavy alcohol consumption in the development of PHC.更多还原