【摘要】 目的:研究DNA-PKcs和Bcl-2在急性白血病(AL)细胞中的表达及相关性。方法:采用免疫组化SP法检测62名初治AL患者骨髓白血病细胞DNA-PKcs和Bcl-2的表达。结果:DNA-PKcs在对化疗药物耐药患者白血病细胞中的表达率(51.5%)明显高于敏感组(20.7%),P<0.05。Bcl-2在对化疗药物耐药患者白血病细胞中的表达率(84.8%)明显高于敏感组(62.1%),P<0.05。耐药组患者的白血病细胞中DNA-PKcs(+)/Bcl-2(+),DNA-PKcs(+)/Bcl-2(-),DNA-PKcs(-)/Bcl-2(+)和DNA-PKcs(-)/Bcl-2(-)细胞的百分率分别是88.2%,33.3%,44.8%和30.0%,DNA-PKcs(+)/Bcl-2(+)与DNA-PKcs(+)/Bcl-2(-)、DNA-PKcs(-)/Bcl-2(+)和DNA-PKcs(-)/Bcl-2(-)之间差异均有统计学意义(P<0.05,P<0.01,P<0.01)。33名耐药组患者中,DNA-PKcs和Bcl-2的表达无相关性,r=0.171,P>0.05。29名敏感组患者中,DNA-PKcs和Bcl-2的表达呈负相关,r=-0.45,P<0.05。结论:DNA-PKcs可能下调Bcl-2的表达,AL患者的白血病细胞中DNA-PKcs和Bcl-2表达水平增高与临床耐药密切相关。更多还原

【Abstract】 OBJECTIVE:To study the expressions of DNA-PKcs and Bcl-2 and their relationship in acute leukemia (AL) patients. METHODS: The expressions of DNA-PKcs and Bcl-2 in bone marrow mononuclear cells of 62 AL patients were measured by SP immunohistochemistry method. RESULTS: The positive expression rate of DNA-PKcs in the treatment resistant group(51.5%)was significantly higher than that in the sensitive group(20.7%),P<0.05. The positive expression rate of Bcl-2 in the treatment resistant group(84.8%)was significantly higher than that in the sensitive group(62.1%),P<0.05. The percentage of patients with DNA-PKcs(+)/Bcl-2(+) in the treatment resistant group(88.2%) was significantly higher than that of ones with DNA-PKcs(+)/Bcl-2(-)(33.3%),DNA-PKcs(-)/Bcl-2(+)(44.8%) and DNA-PKcs(-)/Bcl-2(-)(30.0%), respectively(P<0.05, P<0.01, P<0.01). In the treatment resistant group, there was no correlation between DNA-PKcs and Bcl-2 expression, r=0.171,P>0.05. However, in the treatment sensitive group, negative correlation existed between the expression of DNA-PKcs and Bcl-2, r=-0.45,P<0.05. CONCLUSIONS: DNA-PKcs probably down-regulates Bcl-2 in triggering apoptosis in response to severe DNA damage, and the higher expressions of DNA-PKcs and Bcl-2 are closely correlated with clinical drug resistance.更多还原