【摘要】 目的:探讨大鼠不同剂量脑照射后不同时期神经行为和循环内皮细胞(circu-lating endothelial cells,CECs)数量的变化。方法:108只SD大鼠信封法随机分成3组,用60Co单次照射大鼠脑,A组为空白对照组,B组为10Gy组,C组为30Gy组。Morris水迷宫分别对照射后7d、1个月和6个月的大鼠进行神经行为检测,水迷宫实验结束后心脏取血计数CECs。结果:照射7d后,3组大鼠Morris水迷宫的游泳潜伏期、平台象限时间、穿过平台区域次数差异无统计学意义,P>0.05;照射1和6个月后Morris水迷宫B、C组较A组及C组较B组潜伏期延长,平台象限时间缩短,穿过平台区域次数减少,差异有统计学意义,P<0.05;CECs数量在1周和1个月时B、C组较A组及C组较B组有明显增高,差异有统计学意义,P<0.05;6个月时3组CECs数量之间差异无统计学意义,P>0.05。在B和C组1周时CECs数量和6月时潜伏期相关,rB=0.97,rC=0.95。结论:CECs数量改变与损伤严重程度相关。更多还原

【Abstract】 OBJECTIVE:To investigate the neurobehavior and the circulating endothelial cells (CECs) change in the different time after the different dosages of irradiation on the encephalon of rats. METHODS: One hundred and eight rats were divided into 3 groups in random, Group A was control group, Group B was irradiated by 60Co at a single dose of 10 Gy on the encephalon of rats, Group C was 30 Gy. The neurobehavioral changes were observed by Morris water labyrinth in 7 days, 1 month and 6 months, respectively after the irradiation. The CECs that were isolated from right ventricular blood were counted after Morris water mase test. RESULTS: In 7 days after the irradiation, the swim lantency, the time of swim in the platform quadrant, the time of swim through the plateau region were the same in 3 groups. In 1 month and 6 months after the irradiation, the swim lantency was C>B>A (P<0.05), the time of swim in the platform quadrant and the time of swim through the plateau region were A>B>C, P<0.05. In 7 days and 1 month after the irradiation, CECs were A>B>C >(P<0.05), but in 6 months, there was no difference among the 3 groups. In Group B, CECs in 1 week were correlated with the swim lantency in 6 months (rB=0.966), the same things happened in Group C, rC=0.95. CONCLUSIONS: CECs were correlated with the radiation induced brain injury.更多还原