【摘要】 目的通过研究胰岛素受体(insulin receptor,IR)在链脲佐菌素诱导的糖尿病大鼠模型中心肌组织中的表达,探讨IR与糖尿病性心肌病发病机制的关系。方法成年Sprague-Dawley雄性大鼠20只,成实验组和对照组。实验组用链脲佐菌素诱导产生糖尿病模型,4周后对各组大鼠心肌进行IR免疫组织化学染色,观察IR在糖尿病大鼠和正常大鼠左心室心肌细胞中的表达差异。结果发现糖尿病大鼠中心肌间质纤维增生,心肌间微血管壁增厚,IR表达阳性心肌细胞的数目为(9.42±2.32)个/mm2及其光密度均为(66.58±6.32)较正常对照组减少(t分别=17.24、8.75,P均<0.05)。结论糖尿病心肌病可作为一种特异性的病变发展为心肌自主神经系统的紊乱和冠脉血流储备的障碍。IR表达阳性心肌细胞减少可能会造成心肌糖代谢的损害与心力衰竭的发生。更多还原

【Abstract】 Objective To investigate the expression of insulin receptor(IR)in diabetic model induced by streptozotocin. Methods Twenty adult male Sprague-Dawley rats were divided into two groups at random. In diabetic group, the rats were induced to diabetes by streptozotocin injection. After continuous feed for 4 weeks, these animals were killed and the hearts were taken out. The freeze sections of these tissues were stained with IR immunohistochemistry method. Results Compared with control group, We found fibrosis of myocardial cell, microvessel dissepiment thickening and the densities and numbers of IR immunostaining positive myocardial cell in left ventricle showed a significant decrease in the heart of diabetic rats( t=17.24,8.75, P<0.05). Conclusion Diabetes cardiomyopathy will develop to automatic nervous disorder in myocardium and coronary artery blood current disturbance. The down-regulation of IR in the left ventricle myocardium in diabetic model may relate to pathologic changes of diabetic heart disease, such as myocardial glycometabolism impairment and myocardial failure.更多还原