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活性污泥对聚-β-羟基丁酸酯(PHB)积累能力的影响

Effect of activated sludge on poly-β-hydroxybutyrate(PHB) accumulation

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【作者】 管运涛王乐滕飞常爱敏

【Author】 GUAN Yuntao1,2,WANG Le2,TENG Fei2,CHANG Aimin3(1.Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China;2.Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China;3.Shenzhen Water Quality Monitoring Center,Shenzhen 518001,China)

【机构】 清华大学深圳研究生院清华大学环境科学与工程系深圳市水质检测中心 深圳518055北京100084深圳518001

【摘要】 为了实现活性污泥资源化,用序批式反应器分析典型周期内聚-β-羟基丁酸酯(PHB)含量与化学需氧量(COD)和总磷(TP)浓度的相关性。用聚合酶链式反应与变性梯度凝胶电泳方法对系统中的活性污泥种群结构进行鉴定,并在好氧曝气条件下通过投加高浓度碳源,比较不同PHB含量的活性污泥再次积累PHB的能力。结果表明:COD去除、PHB积累、TP浓度变化符合序批式反应器系统积累PHB的典型模型;转化为PHB的COD比降解的COD多出含碳量1.32mmol/L,存在固定CO2的现象;光合细菌红杆菌属是该系统中的优势种群;PHB含量较低、较高的微生物将COD转化为PHB的效率分别为46.54%和29.04%。

【Abstract】 The poly-β-hydroxybutyrate(PHB) concentration,chemical oxygen demand(COD),and total phosphorous(TP) during one circle were analyzed in a sequenced batch reactor to study the relationship of the PHB,the COD,and the TP.The structure of the activated sludge was identified using polymerase chain reaction and denaturing gradient gel electrophoresis.High concentrations of carbon sources were added under aerobic condition to compare the PHB accumulation capabilities of activated sludge with various PHB levels.The results show that the COD removal,PHB accumulation,and TP change are the same as predicted by the classical PHB accumulation model by sequenced batch reactor.The COD transformed to PHB was 1.32 mmol/L carbon more than the amount of the COD degraded.Photosynthetic bacteria were the dominant microorganism.The activated sludge with low PHB concentration transforms 46.54% of the COD while the high PHB concentration sludge transforms 29.04%.

【基金】 国家自然科学基金资助项目(50108007,50238020,50478011)
  • 【文献出处】 清华大学学报(自然科学版) ,Journal of Tsinghua University(Science and Technology) , 编辑部邮箱 ,2007年06期
  • 【分类号】X703
  • 【被引频次】18
  • 【下载频次】645
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