【摘要】 目的通过体外培养的新生大鼠心肌成纤维细胞研究肝细胞生长因子（HGF）对结缔组织生长因子表达（CTGF）的影响。方法差速贴壁法分离新生SD大鼠心肌成纤维细胞（CFs）及免疫组化法对其进行鉴定,选AngⅡ(10^-6mol/L)作诱导浓度,与HGF100ng/ml孵育48h,采用反转录聚合酶链反应（RT-PCR）技术测定CFsCTGFmRNA的表达,WesternBlot法测定CTGF蛋白的表达。结果肝细胞生长因子能够在mRNA及蛋白水平上大部分抑制AngⅡ对CTGF表达的诱导。结论初步证实在心肌纤维化的过程中,HGF与CTGF是一对拮抗生长因子,HGF可能通过抑制CTGF表达的途径逆转心肌纤维化。更多还原

【Abstract】 Objective To observe the effect of hepatocyte growth factor on the expression of connective tissue growth factor (CTGF) in cultured cardiac fibroblast(CFs).Methods CFs of neonatal Sprague-Dawley (SD)rats were isolated and cultured. Immunocytochemistry staining was used to monitor the marker antigen of the neonatal rat cardiac fibroblasts. HGF 100ng/ml and AngⅡof 10-6mol/L were added to the medium for 48 hours to observe the effects of HGF on CTGF mRNA and protein expression stimulated by AngⅡ.The expression of CTGF mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot respectively.Results HGF could partly reduce the AngⅡ-dependent expression of CTGF both in mRNA and protein level. Conclusion HGF and CTGF may be a couple of contradictory growth factor. HGF could delay the procession of their myocardial fibrosis partly because of inhibiting the expression of CTGF.更多还原