【摘要】 目的探讨针吸细胞学结合PCR检测技术诊断淋巴结结核的效果。方法观察68例体表淋巴结结核的针吸细胞学特征,记录抗酸染色情况并利用PCR技术检测穿刺组织中的结核分枝杆菌的DNA,计算阳性率。结果根据细胞学所见,淋巴结结核以干酪样坏死物、变性的中性粒细胞及少量淋巴细胞多见,而似上皮样细胞及朗汉斯巨细胞较少见;镜检及抗酸染色阳性率58.8%;PCR检测阳性率则高达88.2%。结论由于结核菌发生变异所致非典型性结核病变增多,其针吸涂片细胞学特点也随之改变,仅依赖镜下及抗酸染色漏诊率高;细胞学结合PCR检测技术可大大提高淋巴结结核的阳性检出率。更多还原

【Abstract】 Objective To sum up the outcome of the fine needle aspiration cytology(FNAC) combined with PCR detection for lymphatic tuberculosis.Methods FNAC different features of 68 cases with lymphatic tuberculosis were observed,acid-fast stain and PCR results were recorded,then positive rate was calculated.Results According to the cytological characters,the smears with caseation necrosis ,neutrophil and a few of lymphtic cells appearing at the same time were mostly frequently found.Langhan’s giant cells,epithelioid cells and caseation necrosis were not often appeared at the same time, acid-fast stain positive rate was 58.8%,PCR positive rate was 88.2%.Conclusion Because of the variant acid-fast bacillus and atypical tuberculosis becoming increasingly common,the cytological characteristics on smear are also changing.Routine acid-fast staining would result in missed diagnosis.FNAC combined with PCR could enhance the detection rates of Bacillus tuberculosis.更多还原