【摘要】 目的:探讨细胞角蛋白(CK)5/6和CK34βE12在乳腺导管内增生性病变中的表达及其作为分子标记物在乳腺增生性病变鉴别诊断中的价值。方法:乳腺增生性病变50例,包括普通性增生(UDH)20例,不典型增生(ADH)6例和导管原位癌(DCIS)24例,采用EnVision二步法对其进行CK5/6和CK34βE12的免疫组织化学染色。用SAS统计软件进行统计分析。结果:(1)CK5/6在UDH、ADH、DCIS3组病变中阳性相对数分别为20/20、0/6、0/24;CK34βE12在这3组病变中的阳性相对数分别为20/20、1/6、3/24。(2)UDH与ADH、DCIS在CK5/6、CK34βE12的表达差异均有统计学意义(P均<0.01)。结论:CK5/6和CK34βE12可以作为乳腺UDH和ADH/DCIS鉴别诊断的标记物,虽然CK5/6和CK34βE12表达缺失都提示为ADH/DCIS,但CK5/6的特异性高于CK34βE12,是更为理想的鉴别诊断标记物。更多还原

【Abstract】 Objective:To determine the differential diagnostic value of cytokeratin 5/6 and 34βE12 in the spectrum of proliferative intraepithelial lesions of the breast,including usual ductal hyperplasia(UDH),atypical ductal hyperplasia(ADH),ductal carcinoma in situ(DCIS).Methods:Immunostaining was performed using monoclonal antibodies against CK5/6 and CK34βE12 on 50 paraffin-embedded breast tissue,including UDH(n=21),ADH(n=6),and DCIS(n=24).SAS software was applied to analyze the differential diagnostic value of CK5/6 and CK34βE12 in UDH and ADH/DCIS.Results:The expression of CK5/6 in UDH,ADH and DCIS was found to be20/21,0/6 and 0/24,while the CK34βE12 was 20/21,1/6 and 3/24. The expression of CK5/6 and CK34βE12 were significant difference between UDH and ADH/DCIS(P<0.01,P<0.01). Conclusion: We confirmed that CK5/6 and CK34βE12 are useful in distinguishing UDH from the spectrum of ADH/DCIS,and CK5/6 is more specific than CK34βE12.更多还原