【摘要】 通过L-谷氨酸苄酯和三光气反应制备谷氨酸苄酯的N-羧酸酐,以聚羟乙谷氨酸酰胺为大分子引发剂,投入不同配比的单体/引发剂(A/I),共聚得到两亲性嵌段共聚物聚羟乙谷氨酰胺-聚谷氨酸苄酯(PHEG-PBLG);通过核磁共振技术分析表征该合成聚肽的结构组成、分子量范围及螺旋结构;通过体外酶解实验研究了共聚物的降解情况.研究结果表明:该合成聚肽化合物为两亲性嵌段聚合物:当聚合物中亲水链段PHEG分子量为9000,A/I为75∶1,100∶1,150∶1,200∶1时,相应的共聚物的分子量分别为:1.27×104,1.75×104,1.9×104,3.60×104;该合成聚肽含有α-螺旋结构,随着TFA的加入,该聚肽的结构由α-旋构象转化为随机盘绕构象.更多还原

【Abstract】 Initiated by poly(hydroxylethyl glutamine), amphiphilic block copolymer of poly(hydroxylethyl glutamine (PHEG)) and poly(γ-benzyl-L-glutamate (PBLG)) was synthesized by polymerization of N-carboxyl anhydride (NCA). NCA monomer was synthesized by reacting γ-benzyl-L-glutamate with triphosgene. The constitution, molecular weight and α-helical structures of the resulting block copolymers were studied by NMR and enzymolysis of the black polymers in vitro was discussed. The results showed that the copolymers with different block-ratio of PHEG/PBLG had different molecular weights, being 1.27×104,1.75×104,1.9×104 and 3.60×104 when the A/I ratios were 75∶1, 100∶1,150∶1 and 200∶1, respectively. The copolymers contained α-helix structures in CHCl3, which could be converted into random coils with addition of TFA.更多还原