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放线菌A2菌株代谢产物的研究初报

Preliminary Study on Metabolic Products of Actinomycete A2

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【作者】 黄伟平; 方丽萍; 姬志勤; 龙建友; 邹强; 魏少鹏; 陈华保; 吴文君;

【Author】 HUANG Wei-ping1, FANG Li-ping1, JI Zhi-qin1, LONG Jian-you2, ZOU Qiang1, WEI Shao-peng1, CHEN Hua-bao1, WU Wen-jun1 (1.Inst of Pesticide, Sci Northwest Sci-tech University of Agric & Forest, Yangling 712100, Shaanxi, China; 2.College of Environmene Science and Engineering, Guangzhou University, Guangzhou 510405, China)

【机构】 西北农林科技大学农药研究所; 广州大学环境科学与工程学院; 西北农林科技大学农药研究所 陕西杨凌712100; 陕西杨凌712100; 广州510405;

【摘要】 从渤海海水中分离到一株编号为A2的放线菌,经初步鉴定为链霉菌(Streptomyces)。该菌株产生的代谢产物对许多重要农作物病害具有强烈的抑制作用。采用菌丝生长速率法测定其对小麦赤霉病菌、烟草赤星病菌、番茄灰霉病菌、马铃薯干腐病菌的菌丝生长影响,抑制率达到90%以上;孢子萌发法测定其对烟草赤星病菌、小麦赤霉病菌和马铃薯干腐病菌的孢子萌发的影响,抑制率都在90%以上。盆栽试验结果表明,发酵产物对小麦白粉病的保护和治疗作用分别为98.31%、96.56%。经pH纸色谱和捷克八溶剂系统纸层析实验,初步推定A2所产生的抗生素为碱性水溶性抗生素。

【Abstract】 The fermentation extracets A2 strain of actionmycete isolated from the water of Bo Hai were assayed, and it was indentified to be Streptomyces. The results showed that the fermentation extracts had a high fungicidal activity to mycelium growth or spore germination of Fusarium raminearum, Alternaria longipes, Botrytis cinerea, Fusarium solani, Curvulavia lunata. The pot tests showed that the fermentation extracts exhibited a protective efficacy of 98.31% and a therapeutic efficacy of 96.56% against Pseudoperonospora cubensis. The results of Jack’s Eight Solvent System Chromatography and pH Paper Chromatography of the fermentation extracts from strain A2 indicated that the main active components was basic and water-solubable.

【基金】 国家863高技术计划项目(2002AA245121)
  • 【分类号】S476
  • 【被引频次】15
  • 【下载频次】263
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